| December 7, 2015

A 2-kb fragment of DNA is cloned using PCR, purified, and subjected to differential digestion with the enzymes EcoR1 and HindIII. Gel electrophoresis is used to fractionate the products of each digest and the restriction patterns are visualized by staining the agarose gel with ethidium bromide. Using the results from this experiment (shown below), construct a restriction map of the DNA.

Enzyme used Restriction fragment lengths generated

EcoR1 200bp, 500bp, 1300bp

HindIII 300bp, 1700bp

EcoR1/HindIII 100bp, 200bp, 400bp, 1300bp

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