genetics

| December 8, 2015

Researchers want to determine how regulation of cell division is coupled to development in Drosophila melanogaster (flies). To do this, they clone a cdc2 homolog from flies called cdc2fly. To show this gene is expressed in flies, the researchers conduct a northern blot during different times in development. They analyze 4 different stages shown below.

Lanes: 1-early embryos, 2-pupae 3-adult fly 4-fly heads The top panel is probed for 26S RNA. The bottom panel is probed for cdc2fly.

A) Describe the steps for how to conduct a northern blot.

B) What components provide:

Separation?

Specificity?

Detection?

C) Describe the results that you see in each lane for cdc2fly.

D) Describe the results that you see in each lane for the 26S RNA.

Lane 1-4 has same size, same intensity bands for the 26S RNA. It is used as a control, as it is equally present and expressed.

E) Given what you have learned about the role of the cdc2 protein and the types of tissues examined, what is the conclusion you can draw from the data in this experiment (both panels)?

26S RNA (ribosomal RNA) are highly conserved between species, which is good to use.

Northern Blot detects RNA, checking transcription. We are looking at whether the gene is being transcribed. 26S RNA is transcribed in every tissue.

G) What alternative approaches can be used to examine RNA expression? What components provide separation, specificity, and detection?

H) Describe an approach to examine protein expression. What components provide separation, specificity, and detection?

I) What would a southern blot show? What components provide separation, specificity, and detection?

Italicized text is my answering attempt.

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