| December 8, 2015

A strain of E. coli has the following mutant genotype: lacZ- gyrA- hisA- (mutations in all three genes). Indicate by a yes or no in the answer space whether the strain would grow on minimal media with the carbon sources (maltose, lactose or glucose) and supplements as indicated below. Lactose and maltose are sugars and histidine is an amino acid. One of these three mutations leads to resistance to an antibiotic.

1. Minimal + maltose + histidine
2. Minimal + lactose + histidine
3. Minimal + glucose + histidine
4. Minimal + lactose + nalidixic acid
5. Minimal + glucose + histidine +nalidixic acid

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