| December 7, 2015

You have isolated an antibiotic, named edeine, from a bacterial culture. Edeine inhibits protein synthesis but has no effect on either DNA synthesis or RNA synthesis. When added to a reticulocyte lysate, edeine stops protein synthesis after a short lag, as shown in the figure below. By contrast, cycloheximine stops protein synthesis immediately. Analysis of the edeine-inhibited lysate by density-gradient centrifugation showed that no polyribosomes remain at the time protein synthesis had stopped. Instead, all of the globin mRNA accumulated in an abnormal 40S peak, which contained equimolar amounts of small ribosomal unit and initiator tRNA.

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