cell biology

| December 3, 2015

In order to study cell cycle regulation, researchers need tools to evaluate what phase of the cell cycle a cell is in. One tool is to stain the DNA in cells with propidium iodide (PI) and use flow cytometry to count the number of cells with different PI staining intensity. PI staining intensity will vary at different phases of the cell cycle to to the differences in DNA content/cell. You are working with a drug that should arrest the cell cycle prior to completion of M phase (metaphase-anaphase transition). You expect the following results compared to control untreated cells if your drug works:

Answer
A. Control cells will have 60% cells with PI staining at an intensity of 100, 10% at 110-190 and 30% at 200. Treated cells will have 100% at 110-190
B. Control cells will have 60% cells with PI staining at an intensity of 100, 10% at 110-190 and 30% at 200. Treated cells will have 100% at 200.
C. Control cells will have 60% cells with PI staining at an intensity of 100, 10% at 110-190 and 30% at 200. Treated cells will have 100% of cells at 100.
D. Control cells will have 100% at 200. Treated cells will have 60% cells with PI staining at an intensity of 100, 10% at 110-190 and 30% at 200.

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