cell biology

| December 7, 2015

After treating cells with a chemical mutagen, you isolate two mutants. One mutant has alanine and the other has methionine at a site in the protein that normally contains valine. After treating the two mutants with the mutagen again, you isolate mutants from each that now have threonine at the original site. Assuming that all mutations involve single-nucleotide changes, deduce the codons that are used for valine, methionine, threonine and alanine at the affected site. Would you expect to be able to isolate a valine-to-threonine mutant in one step?

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